Biodiversity and assembly processes of soil fungal communities in Chinese subtropical forests with variable tree diversity

In der vorliegenden Dissertation wurde der Einfluss der Baumdiversität auf die Bodenpilzgemeinschaft in Chinesischen subtropischen experimentellen Waldflächen untersucht

Experimental Evidence of Functional Group-Dependent Effects of Tree Diversity on Soil Fungi in Subtropical Forests

Deconvoluting the relative contributions made by specific biotic and abiotic drivers to soil fungal community compositions facilitates predictions about the functional responses of ecosystems to environmental changes, such as losses of plant diversity, but it is hindered by the complex interactions involved. Experimental assembly of tree species allows separation of the respective effects of plant community composition (biotic components) and soil properties (abiotic components), enabling much greater statistical power than can be achieved in observational studies. We therefore analyzed these contributions by assessing, via pyrotag sequencing of the internal transcribed spacer (ITS2) rDNA region, fungal communities in young subtropical forest plots included in a large experiment on the effects of tree species richness. Spatial variables and soil properties were the main drivers of soil fungal alpha and beta-diversity, implying strong early-stage environmental filtering and dispersal limitation. Tree related variables, such as tree community composition, significantly affected arbuscular mycorrhizal and pathogen fungal community structure, while differences in tree host species and host abundance affected ectomycorrhizal fungal community composition. At this early stage of the experiment, only a limited amount of carbon inputs (rhizodeposits and leaf litter) was being provided to the ecosystem due to the size of the tree saplings, and persisting legacy effects were observed. We thus expect to find increasing tree related effects on fungal community composition as forest development proceeds

Toward a methodical framework for comprehensively assessing forest multifunctionality

Biodiversity-ecosystem functioning (BEF) research has extended its scope from communities that are short-lived or reshape their structure annually to structurally complex forest ecosystems. The establishment of tree diversity experiments poses specific methodological challenges for assessing the multiple functions provided by forest ecosystems. In particular, methodological inconsistencies and nonstandardized protocols impede the analysis of multifunctionality within, and comparability across the increasing number of tree diversity experiments. By providing an overview on key methods currently applied in one of the largest forest biodiversity experiments, we show how methods differing in scale and simplicity can be combined to retrieve consistent data allowing novel insights into forest ecosystem functioning. Furthermore, we discuss and develop recommendations for the integration and transferability of diverse methodical approaches to present and future forest biodiversity experiments. We identified four principles that should guide basic decisions concerning method selection for tree diversity experiments and forest BEF research: (1) method selection should be directed toward maximizing data density to increase the number of measured variables in each plot. (2) Methods should cover all relevant scales of the experiment to consider scale dependencies of biodiversity effects. (3) The same variable should be evaluated with the same method across space and time for adequate larger-scale and longer-time data analysis and to reduce errors due to changing measurement protocols. (4) Standardized, practical and rapid methods for assessing biodiversity and ecosystem functions should be promoted to increase comparability among forest BEF experiments. We demonstrate that currently available methods provide us with a sophisticated toolbox to improve a synergistic understanding of forest multifunctionality. However, these methods require further adjustment to the specific requirements of structurally complex and long-lived forest ecosystems. By applying methods connecting relevant scales, trophic levels, and above? and belowground ecosystem compartments, knowledge gain from large tree diversity experiments can be optimized

Biodiversity and assembly processes of soil fungal communities in Chinese subtropical forests with variable tree diversity

In der vorliegenden Dissertation wurde der Einfluss der Baumdiversität auf die Bodenpilzgemeinschaft in Chinesischen subtropischen experimentellen Waldflächen untersucht

Biodiversity and assembly processes of soil fungal communities in Chinese subtropical forests with variable tree diversity

In der vorliegenden Dissertation wurde der Einfluss der Baumdiversität auf die Bodenpilzgemeinschaft in Chinesischen subtropischen experimentellen Waldflächen untersucht

Linking Soil Fungal Generality to Tree Richness in Young Subtropical Chinese Forests

Soil fungi are a highly diverse group of microorganisms that provide many ecosystem services. The mechanisms of soil fungal community assembly must therefore be understood to reliably predict how global changes such as climate warming and biodiversity loss will affect ecosystem functioning. To this end, we assessed fungal communities in experimental subtropical forests by pyrosequencing of the internal transcribed spacer 2 (ITS2) region, and constructed tree-fungal bipartite networks based on the co-occurrence of fungal operational taxonomic units (OTUs) and tree species. The characteristics of the networks and the observed degree of fungal specialization were then analyzed in relation to the level of tree species diversity. Unexpectedly, plots containing two tree species had higher network connectance and fungal generality values than those with higher tree diversity. Most of the frequent fungal OTUs were saprotrophs. The degree of fungal specialization was highest in tree monocultures. Ectomycorrhizal fungi had higher specialization coefficients than saprotrophic, arbuscular mycorrhizal, and plant pathogenic fungi. High tree species diversity plots with 4 to 16 different tree species sustained the greatest number of fungal species, which is assumed to be beneficial for ecosystem services because it leads to more effective resource exploitation and greater resilience due to functional redundancy